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Western blotting of total protein showed that levels of the cellular senescence-associated pathway proteins p53, Ser15-phospho-p53, and p21, which are signaling molecules downstream of FOXO4, were increased in the senescent cells (Figure 3F–3I), whereas levels of the testosterone synthesis-related proteins CYP11A1 and CYP17A1 were decreased (Figure 3J–3L)

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