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In a 6 multi-well plate, 1.5 × 10 5 cells were plated and transfected with 900 ng of pHIV-dTomato and 100 ng of pIRES2-EGFP (Clontech #6029-1, Mountain View, CA, USA), a transfection efficiency control, using Lipofectamine 3000 Transfection Reagent (Invitrogen, Life Technologies)
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Therefore, future work will focus on the further isolation of AF compounds, structural analysis, and exploration of their apoptosis‐inducing pathways
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