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Introduction Cryopreservation of the mammalian brain has been studied or proposed as a method of analyzing the metabolism of single cells within the intact brain using the 2-deoxyglucose method [[1]], studying the tolerance of the brain to ischemia [[2]], studying the resistance of whole mammals to freezing [[3, 4]], mapping the connectome and facilitating other neurobiological techniques [[5]], advancing basic cryobiological research [[6]], improving brain banking [[7]], and developing medical applications [[8–11]]
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