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Human HaCaT keratinocyte cells (1×10 5 cells/ml, obtained from Cell Bank of Kunming Institute of Zoology, Chinese Academy of Sciences) were cultured in 96-well plates with Dulbecco’s modified Eagle’s medium (DMEM, Gibco) containing 10% fetal calf serum and penicillin (100 u/ml)-streptomycin (100 mg/ml) at 37°C in a humidified 5% CO 2 atmosphere

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Sikiric and colleagues, who have authored much of the foundational BPC-157 research, repeatedly describe an interaction with the L-arginine-NO pathway